cytokeratin 6 antibody anti-Cytokeratin 6 antibody [SQab18113] is a Recombinant Rabbit Monoclonal antibody [SQab18113] recognizes Cytokeratin 6, which can be used for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation testing with Human,Mouse,Rat samples. Immunofluorescence: Rat endometrial epithelial (REE) cultured cells fixed with neutral buffered formalin for 10 min, and permeabilized with cold-methanol at -20°C for 10 min and then stained with ARG62969 anti-Cytokeratin (pan) antibody [C-11]. Mod Pathol 2000;13:107-12. Rabbit Cytokeratin 5 Rabbit mAb (A11396), validaed in WB,, IHC,, IF and tested in Human,, Mouse,, Rat. This antibody reacts with human samples. Mouse anti Human cytokeratin type II antibody, clone AE3 recognizes human cytokeratin type II and reacts with most of the Basic (Type II) keratins. Immunohistochemistry for napsin A, Cytokeratin 5/6, p63, and TTF-1 was performed on a formalin-fixed tissue microarray obtained from Toyama, Japan. Antibodies Promotion  31 Mar 2020 IL-6 is an inflammatory cytokine that protects against infection but can lead to cytokine storms. A specific band was detected for Cytokeratin 18 at approximately 55 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619). Synonyms. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. IL-6 antibody Mouse Monoclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF),Enzyme-linked Immunosorbent Assay (ELISA) applications. This dis-Table4 Stainingofnon-epithelial tumours CAM52 AE1/AE3 lu-S MFN116 Cytokeratin-18 Melanoma 0/7 0/7 0/7 0/7 0/7 Lymphoma 0/4 0/4 0/4 0/4 0/4 Malignantfibroushistiocytoma 0/3 0/3 0/3 0/3 0/3 Leiomyoma 0/6 0/6 0/6 0/6 0/6 9. 1. Cytokeratin 6 antibody LS-C392262 is an unconjugated mouse monoclonal antibody to Cytokeratin 6 (KRT6 / CK6) (aa545-555) from human. Immunogen. Concentrated and Prediluted Monoclonal Antibody 901-105-040819 Rev: 062117 Intended Use: For In Vitro Diagnostic Use Cytokeratin 5/6 [CK5/6. These include epithelial cells that are ectodermal, mesodermal, or endodermal in origin. It is reactive with human, horse and sheep. Storage Buffer Mouse Monoclonal Cytokeratin 6 antibody [KRT6A/2368]. By Rodney T. WB, 0. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Rabbit [KO Validated] Cytokeratin 8 Rabbit pAb (A1024), validaed in WB,, IHC,, IF and tested in Human,, Mouse. Tested in IHC applications. Thus in immunoblotting, the antibody labels a number of discrete bands ranging from 40 to 58 kDa, corresponding to cytokeratins 5, 6, 8, 17 and probably 19. The antibody does not cross-react with cytokeratins 1, 7, 8, 10, 13, 14, 18 and 19. It crossblocks Cam5. The sections were then incubated with rabbit anti-GFP antibody (1:1000; Abcam) and mouse anti-pan cytokeratin antibodies (1:200; Abcam) in TBS-T and 10% NGS overnight at 4°C. In adult tissues cytokeratin 8 is expressed as a heterodimer with cytokeratin 18. Anti-Cytokeratin 5, 6, clone D5/16B4 (50mg) KC Antibody Anti-Cytokeratin 5 Antibody, 6, clone D5/16B4 is an antibody against Cytokeratin 5 for use in IF, IH(P) & WB - Find MSDS or SDS, a COA, data sheets and more information. - Find MSDS or SDS, a COA, data sheets and more information. Clone: 34βE12. RRID: AB_2842207 Please cite this product as: Affinity Biosciences Cat# DF9011, RRID:AB_2842207. This MAb recognizes a protein of 56 kDa, identified as cytokeratin 6 (CK6). A specific band was detected for Cytokeratin 18 at approximately 48 kDa (as indicated). 1 Product Result Anti-Cytokeratin 5 & 6 is a marker for eptihelioid mesotheliomas. Cytokeratin 6 Antibody (MA5-16373) in WB Western blot analysis of A431 Cells using anti-Keratin 6 Monoclonal Antibody (Product # MA5-16373). Cytokeratin 5/6 (CK 5/6) is a very useful antibody in diagnostic Surgical Pathology. This antibody may also react to bovine, mouse, or rat Cytokeratin, as predicted by immunogen homology. Cytokeratin 5/6 cannot identify mesothelioma on its   Thermo Scientific™ Lab Vision™ Cytokeratin 5/6 Ab-2, Mouse Monoclonal Antibody 12mL; Unlabeled; Ready to Use Primary Antibodies - Alphabetical OverviewProduct Name Cytokeratin 5/6 antibody Product Number IR226-865 Gene Description keratin 5, type IIClonality Polyclonal Host Rabbit Species  . Validated in WB, ICC/IF, IHC-P. Similarly, loss of tumor suppressor gene PTEN and a high expression of c-Met has been associated with poor prognosis Immunohistochemistry with cytokeratin 5 and cytokeratin 6 antibodies on sections from dorsal skin tetop-LA G608G+; K5tTA + F1 line VF1-07 with severe epidermal hyperplasia show mislocalized keratin 5 and keratin 6 expression . Our Boster Quality Guarantee for Anti-Cytokeratin 6 KRT6A Monoclonal Antibody covers its use in the following applications. Tested in Human. Before staining, antigens were retrieved using heat-induced epitope retrieval ( HIER) at pH 9. Cytokeratin clone OSCAR (Signet, Dedham, MA), and CK5/6 produce the strongest and most diffuse immunoreactivity. It has been reported that CK5/6 is a more useful antibody than CK34betaE12 for differentiating DCIS from intraductal papilloma. The exact sequence is proprietary. 1X10^6 HeLa cells were stained with 0. - Find MSDS or SDS, a COA, data sheets and more information. We offer a wide range of validated Cytokeratin 18 antibodies. As originally reported, IL-6 helps activated B cells to differentiate into antibody-producing cells, and it also promotes the growth of myelomas/plasmacytomas and enhances the survival of plasmablasts (13, 14). This antibody reacts with human, mouse samples. 0) subfamilies. 1X10^6 HeLa cells were stained with 0. , Yi, C. NCL-CK5/6/8/18, NCL-L-CK5/6/8/18 and RTU-CK5/6/8/18 react with human cytokeratins 5, 6, 8 and 18. Order online or by email, fax, or phone. Common antibodies are directed against both cytokeratin 5 and 6 Major partner of CK5 is CK14, while CK6 pairs with CK16/17 ( Exp Cell Res 2007;313:2244 , Eur J Cell Biol 2004;83:735 ) CK5 also detected by antibodies against high molecular weight cytokeratins (such as 34βE12, also known as K903) and pan-CK markers (such as CK AE1/AE3) Compare Anti-Keratin 6 Antibody Products from leading suppliers on Biocompare. No. Concentration: 1mg/ml. Reactivity: Human. 100% Guaranteed. This experiment was conducted under reducing Cytokeratin 14 Antibody detects endogenous levels of total Cytokeratin 14. The antibody does not cross-react with cytokeratins 1, 7, 8, 10, 13, 14, 18 and 19. No reactivity with other mesodermally derived tissues, such as muscle and connective tissues, has been observed. com | A121367 Twenty identified cytokeratins make up a complex family of intermediate filaments. 4 reviews. 2,5,6 Clone The Anti-Cytokeratin antibody, clone CAM 5. RM226 reacts to human CK-5 (Cytokeratin 5), and does not cross-react to CK-6 (Cytokeratin 6). The cells were blocked with 10% goat serum. 2, is derived from the hybridization of P3/NS-1/1-Ag4-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with the human colorectal carcinoma cell line HT29. Fixed with 90% MeOH blocked with 3% BSA (30 min). Boster Bio Anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal Antibody catalog # M30943. After they were rinsed in TBS, the sections were incubated in TBS-T and 10% NGS containing goat anti-rabbit Alexa Fluor 488 and goat anti-mouse Alexa Fluor 546 (1:200 With regard to the staining pattern of specific antibodies, cytokeratin 5/6 was positive in 2 of 7 metastatic carcinomas from the female genital tract, 3 of 10 metastatic colonic carcinomas, but IHC Select Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3 is an antibody against Cytokeratin AE1/AE3 (Pan cytokeratins) for use in IH(P). Fewer than 10% of Carcinomas of the breast, colon, and prostate stain positively for this marker. Antibodies. P48668, P04259 and P02538 100% homology. AE1 detects the high molecular weight cytokeratins 10, 14, 15, and 16, and also the low molecular weight cytokeratin 19 Anti­Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619) followed by HRP­conjugated Anti­Mouse IgG Secondary Antibody (Catalog # HAF018). One of the most important aspects of this marker is that it characteristically stains squamous carcinomas strongly and diffusely, but generally stains adenocarcinomas focally, weakly, or not at all. CK 5 and 6 are positively seen in nearly 100% of Malignant Mesotheliomas and are rarely seen in Lung Adenocarcinomas. 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). 1 mg/mL 100 or 500 uL Cytokeratin 6 Cytokeratin 7 Cytokeratin 8 Cytokeratin 8/18 R-PLEX Human Cytokeratin-8 Antibody Set The R-PLEX platform offers matched sets of antibodies and calibrators for a wide menu of biomarkers. Return to top. Cells were then stained with pan-Cytokeratin Polyclonal Antibody(bs-2190R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. 1 Product Result 31-1105-00. '(6&5,37,21 6SHFLHV5HDFWLYLW\ Human 6SHFLILFLW\ Detects human Cytokeratin 18 in direct ELISAs and Western blots. Cytokeratin 5/6 antibodies are antibodies that target both cytokeratin 5 and cytokeratin 6. Anti-mIL-6-IgG is a monoclonal antibody specific for murine interleukin 6 (mIL-6). 6, supplemented with 0. Identification of the Substance/Mixture and of the Company/Undertaking Cytokeratin 20 IT-Ks20. More>> Anti-Cytokeratin 10 Antibody, clone RKSE60 detects level of Cytokeratin 10 & has been published & validated for use in FC, WB, IC, IH. D. Immunohistochemistry with cytokeratin 5 and cytokeratin 6 antibodies on sections from dorsal skin tetop-LA G608G+; K5tTA + F1 line VF1-07 with severe epidermal hyperplasia show mislocalized keratin 5 and keratin 6 expression . Product Data Sheet. CK 5 and 6 can positively be seen in Fig. Supplied as 150 µL purified antibody (0. To learn more comprehensive our antibody product information including immunogen, specificity, and more, you can read all details here. Supplied as 100ug/vial in Liquid form antibody. Cells were fixed with 90% MeOH. References 1. Clone REA885 recognizes the human type II keratin protein cytokeratin 8, an intermediate filament protein, which is found in epithelial and carcinoma cells. com for all your antibody needs. Validated in WB, IHC-P, IHC-Fr. We would therefore not usually offer a replacement antibody as this requires that the complaint be made within 6 months of the purchase of the antibody to comply Cite Cytokeratin 5/6 Monoclonal Antibody (D5/16 B4) The following product was used in this experiment: Cytokeratin 5/6 Monoclonal Antibody (D5/16 B4) from Thermo Fisher Scientific, catalog # MA1-91106, RRID AB_1955731. Of these, high-molecular weight cytokeratins (CKs) traditionally expressed by the basal epithelial/myoepithelial cells of normal breast, including those detected by the 34 β E12 antibody (CK1, CK5, This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. BioLegend’s anti-cytokeratin antibody clone OSCAR demonstrates a broad spectrum of cytokeratin reactivity. Tested Reactivity: Human. The following lists a number of Pan cytokeratin antibodies from various sources. It has also been shown to react with cytokeratin 6, weakly with cytokeratin 4, and does not react with cytokeratins 1, 7, 8, 10, 13, 14, 18 and 19. To learn more comprehensive our antibody product information including immunogen, specificity, and more, you can read all details here. However, Cytokeratin 7 is absent from gastrointestinal epithelium Primary Antibodies. 0 was used for antibody retrieval(>98°C,20min). 16853-1-AP. Validated for IHC and WB. 1, 5, 6, 7, and 8) of the 'basic cytokeratin subfamily' defined by peptide mapping, as well as a few acidic cytokeratins such as the epidermis-specific cytokeratins Nos. After labeling the antigen with a primary In subgroups of breast cancer, the shortest disease-free and overall survival was observed in basaloid and human epidermal growth factor receptor-2 groups. For breast pathology, also in distinguishing usual ductal hyperplasia (UDH) and papillary lesions (having a mosaic-like pattern) from ductal carcinoma in situ, which is usually negative. Western Blot analysis of Cytokeratin 14 using anti-Cytokeratin 14 Polyclonal Antibody (PA5-28002), in Hela Cell Lysate (30 ug of cell lysate) and at a dilution of 1:3000. ERG is expressed at higher levels in early myelocytes than in mature lymphocytes, and thus ERG may act as a regulator of differentation of early hematopoietic cells. Cytokeratin 5/6 antibodies are antibodies that target both cytokeratin 5 and cytokeratin 6. 1ml or 5ul per 100ul of whole blood); Immunofluorescence (1:50-1:100 for 30 minutes at RT); Optimal dilution for a specific application should be determined. The anti-Cytokeratin 6 Rabbit Monoclonal antibody (M30943-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. , 2016), SOX10/S100 as a melanoma marker (Mohamed et al. The anti-Keratin sample set provides antibodies directed against Keratin K17, K18, K19, K8/18 and pan to evaluate the presence and status in IHC and WB. 5 ug Control Antibody. Y. 1 Product Code: 10349 Section 1. Bai H Separation of HeLa cells stained using anti-cytokeratin 18 (C-04) purified antibody (concentration in sample 0,6 μg/ml, GAM FITC, red-filled) from HeLa cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension. Cytokeratin 6 (56 kDa) is also a high molecular weight, basic type cytokeratin hepatocellular and renal cell carcinomas. Clone Name. Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2 recognizes the rod domain (aa 312-335) of human cytokeratin 19 ( Antibody [M7237] Cytokeratin 5/6 (Concentrate) by Dako. In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. (PA5-16722) Cytokeratin 14 Polyclonal Antibody - Invitrogen Antibodies - CiteAb. In direct ELISAs, less than 1% cross­reactivity with recombinant human Cytokeratin 14 (KRT14) is observed. No cross-reaction with cytokeratins 1, 7, 8, 10, 13/14, 18 and 19 has been found. 1X10^6 HeLa cells were stained with 0. Cytokeratin 5/6 (Autostainer Link 48) Clone D5/16 B4. Be the first to share your experience with this product. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Cytokeratin 5 is expressed in various epithelia and mesothelial cells, while cytokeratin 6 is expressed by proliferating squamous epithelium. It labels mesothelioma, and epithelial basal cells in prostate and tonsil. Almost a year prior to the report of the complaint. The section was stained using Cytokeratin 5/6/8/17/19 Antibody, anti-  Product Specific Information. Millipore’s Cytokeratin antibodies demonstrate specificity against cytokeratin. IL-6 is also important for adaptive immune responses by promoting B- and T-cell differentiation. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. 1: Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, 5/6/18 Mouse Monoclonal Antibody (SPM267). Four of five of the ADH cases showed less than 5% of CK5/6 stained cells, the remaining case showed 30% of labeled cells. 1 Cytokeratin 5 (58kDa) & cytokeratin 6 (56kDa) are type II high molecular weight keratins that are expressed in a broad range of normal tissues including breast, prostate, mesothelium, skin and esophagus. Antigen Background. CK8/18 [5D3] recognizes Cytokeratin 8 and 18 intermediate filament proteins. This rabbit monoclonal antibody [clone BC1] is highly specific to cytokeratin 7 and shows no cross-reaction with other IFP’s. Immunohistochemistry-Paraffin: Cytokeratin 6 Antibody (SPM269) - Azide and BSA Free [NBP2-34406] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Cytokeratin 6 Monoclonal Antibody (SPM269). Reacts strongly with cytokeratins 5 and 6 and weakly with cytokeratin 4. Including epidermal cell keratin of ~86 and 58 kDa and skin at ~58 kDa, 65 kDa and 67 kDa. Specificity. Both of these individual clones detect certain high and low molecular weight keratins. It also labels cytokeratin 6 and weakly labels cytokeratin 4. Cytokeratin 5 & 6 antibody has also been used successfully Buy cytokeratin 6 antibodies from Santa Cruz Biotechnology, Inc. Key Applications: Immunocytochemistry; Application Notes: The unconjugated antibody (Cat. Similarly, loss of tumor suppressor gene PTEN and a high expression of c-Met has been associated with poor prognosis mouse Interleukin 6 IL6 antibody validated for ELISA,Western Blot Javascript is disabled on your browser. 2 Union Square, Union City, CA 94587. Clover J, et al. Cytokeratin 6A antibody Mouse Monoclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF),Enzyme-linked Immunosorbent Assay (ELISA) applications. It is reported that an enrichment of up to 10,000 times of desired cells can be achieved by this method. View specifications, prices, citations, reviews, and more. In normal tissues, [5D3] recognizes all simple and glandular Western blot analysis of Cytokeratin 5+6 on A431 cell lysate. The Anti-Cytokeratin antibody might not react with some squamous cell carcinomas. In Western blotting of cytoskeletal preparations of epidermis and non-keratinizing epithelium, the antibody labels cytokeratin 5. Replacement to Abcam, Santa Cruz, Sigma and CST antibody. Immunohistochemistry-Paraffin: Cytokeratin 6 Antibody (LHK6 (same as LHK6B)) [NBP2-34232] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Cytokeratin 6 Monoclonal Antibody (LHK6). Description. NCL-L-CK5/6/8/ 18 is a cocktail of monoclonal antibodies designed to recognize all epithelial  Using the pan-cytokeratin, antibody-positive immunoreaction of single or CK5/ 6 often stains the fusiform, poly-gonal to plump spindle cells but does not stain  Human Prostate Carcinoma stained with anti-Keratin 6 antibody. Host: Mouse ENZ-30903, 6 ml, ~6 µg, 204. TintoFast Cytokeratin 5 & 6 are positively seen in nearly 100% of Malignant Mesotheliomas and is rarely seen in Lung Adenocarcinomas. 007] is a mouse monoclonal antibody that is intended for laboratory use in the qualitative identification of cytokeratin 5 and 6 proteins by immunohistochemistry (IHC) in formalin-fixed Mouse Monoclonal Cytokeratin 6 + 18 antibody [LP34]. It is not intended for diagnostic or therapeutic use. We evaluated p40 expression and compared it with the exp … p40 is the best marker for diagnosing pulmonary squamous cell carcinoma: comparison with p63, cytokeratin 5/6, desmocollin-3, and sox2 MAB3224 is a broadly reacting cytokeratin antibody which reacts with cytokeratin 5 among others. Fixed with 90% MeOH blocked with 3% BSA (30 min). These products are cocktails of monoclonal antibodies designed to recognize cytokeratins reported to be expressed in almost all epithelial tissues. 8 Cytoplasm AM315, AX315, MU315 Cytokeratin 20 EP23 Cytoplasm AN849, AY849, NU849 Cytokeratin 7 Cytokeratin 7 is a 54 kD marker of simple epithelium. Cytokeratin MNF116 is a broad-spectrum anti-cytokeratin reacting with intermediate and low-molecular-weight keratins, ranging from 40 through 58 kD, corresponding to cytokeratin 5, 6, 8, 17 and 19. 1 Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. References. 736111111111111 *The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user. To view this site, you must enable JavaScript or upgrade to a JavaScript-capable browser. 13) binds to a determinant present in a large number of human cytokeratin polypeptides, notably some polypeptides (Nos. Cytokeratin 19 in MCF‑7 Human Cell Line. Validated in IHC-P. 2015/830 and US OSHA HCS 2015 1. Distribution of cytokeratins and vimentin in adult renal neoplasms and normal renal tissue: potential utility of a cytokeratin antibody panel in the differential diagnosis of renal tumors Am J Surg Pathol . Cytokeratin 19 in MDA‑MB‑231 Human Cell Line. The Cell Marque Cytokeratin 5 & 6 (D5 & 16B4) antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods. 18 and 19. Levels Indicate Exposure. This MAb recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor® 488 is an antibody against Cytokeratin 5,6 for use in Immunocytochemistry. This antibody reacts with Human samples. Cytokeratin 6 antibody detects Cytokeratin 6 protein at membrane on mouse cervix by immunohistochemical analysis. Cytokeratin 6 Antibody (LHK 6) is a mouse monoclonal IgG 2a κ, cited in 4 publications, provided at 200 µg/ml raised against a synthestic peptide of Cytokeratin 6 of human origin recommended for detection of Cytokeratin 6 of mouse, rat and human origin by WB, IP, IF and IHC(P) Flow Cytometry - Anti-Cytokeratin 6 antibody [Ks6. Monoclonal Anti-Cytokeratin, pan−FITC antibody produced in mouse. Cytokeratins are known markers of tissue differentiation and are used to determine the malignancy of tumors. Replacement to Abcam, Santa Cruz, Sigma and CST antibody. The cytokeratins are encoded by a family encompassing 30 genes. In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. Milli-Mark™Anti-Cytokeratin 5 Antibody, 6-FITC, clone D5/16B4 Milli-MarkAnti-Cytokeratin 5 Antibody, 6-FITC, clone D5/16B4 is an antibody against Cytokeratin 5 for use in FC. Western blot analysis of Cytokeratin 19 on MCF-7 cell lysates. In subgroups of breast cancer, the shortest disease-free and overall survival was observed in basaloid and human epidermal growth factor receptor-2 groups. 6% and 78%) than the CK phenotype. Last; Cytokeratin 5 & 6 EP24 & EP67 Cytokeratin 5 (EP1601Y) + Cytokeratin 14 (LL002) Cytokeratin 5 & 6 (D5/16B4) + TTF-1 (8G7G3/1) Cytokeratin 7. - Find MSDS or SDS, a COA, data sheets and more information. These antibodies bind and isolate/enrich tumor cells that display cytokeratin 7/8. Immunohistochemical staining of Cytokeratin 6 in human cervix using Cytokeratin 6 Polyclonal Antibody (Product # PA5-61074) shows strong positivity in squamous epithelial cells. Compare cytokeratin Antibody Products from leading suppliers on Biocompare. ️ Low Prices ️ 100% Guarantee ️ FREE Shipping Paraformaldehyde-fixed, paraffin embedded Human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6. Cytokeratin 4+5+6+8+10+13+18 antibody was purified by Protein A Chromatography Immunogen Cytokeratin 4+5+6+8+10+13+18 antibody was raised in Mouse using Keratin preparation from human cultured cell line A431 as the immunogen. 10 and 11 and the more widespread squamous carcinomas to which the antibody was applied. Disclaimer Summary Cytokeratin 5 (58 kDa) is a high molecular weight, basic type of cytokeratin expressed in basal, intermediate and superficial cell layers of stratified epithelia as well as transitional epithelia, complex epithelia and in mesothelial cells and mesothelioma. Validated in IHC-P, FACS. Reacts with Human. Pathologists use cytokeratin 5 to distinguish mesothelioma from adenocarcinoma, the most common type of lung cancer. 1997 Aug;31(2):140-3. Compare Anti-Cytokeratin 5 / 6 Antibody Products from leading suppliers on Biocompare. This antibody is effective in immunohistochemistry (IHC). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. ,et al. Wash in 0. Anti-Cytokeratin 14/KRT14 Rabbit Polyclonal Antibody (100129-RP02), manufactured by Sino Biological is validated in WB,IHC-P. MA1-26237 has been successfully used in immunohistochemistry (paraffin) procedures. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), and immunocytochemistry. , et al. Monoclonal Anti-Cytokeratin, pan−FITC antibody produced in mouse. CDX2 expression at both cut-off levels (> 5% and > 50%) had a higher sensitivity (96. The target antigen refers to the Pan cytokeratin family of proteins, which includes several individual genes. Sample: Paraffin-embedded mouse cervix. Flow Cytometry (5ul per test per one million cells in 0. Immunotag™ Cytokeratin 6 Monoclonal Antibody(4A1) Cytokeratin 6 (56 kD) is also a high-molecular weight, basic type cytokeratin . Cytokeratin 7 is an intermediate filament protein (IFP) of 54 kDa that recognizes the simple epithelium found in most glandular and transitional epithelia; but not that which is found in the stratified squamous epithelia. This antibody exhibits a cytoplasmic staining pattern and may be used to aid in the identification of squamous cell carcinoma and in distinguishing malignant mesothelioma from lung adenocarcinomas. The set contains enough antibody to perform stainings on 6-12 sections per antibody. The Anti-Cytokeratin (CK3-6H5) antibody is a pan-cytokeratin–specific antibody targeting cytokeratins from simple epithelia. ✔️ 100% Guarantee ✔️ FREE   Reacts strongly with cytokeratins 5 and 6 and weakly with cytokeratin 4. This antibody exhibits a cytoplasmic staining pattern and may be used to aid in the identification of squamous cell carcinoma and in distinguishing malignant mesothelioma from lung adenocarcinomas. Key Applications: Immunohistochemistry (Paraffin) Application Notes: Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues. Alternative Name: HMWCK, High molecular weight cytokeratin, Keratin-903. 1907/2006 as amended by (EC) No. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Anti-TTF-1 stains the nuclei in the case of lung adenocarcinomas and is negative in nearly all mesotheliomas. CK5/6 often stains the fusiform, poly-gonal to plump spindle cells but does not stain thin spindled cells. Cytokeratin 7 and Cytokeratin 20 expression have been reported to show characteristic patterns on primary and metastatic lung and colorectal adenocarcinomas. Validated: WB, Flow, IHC, IHC-P. Alternative Names (click to expand) anti CK 12 antibody, anti CK12 antibody, anti Cytokeratin 12 antibody, anti K12 antibody, anti keratin 12 (Meesmann corneal dystrophy) antibody, anti Keratin 12 antibody, anti Keratin, type I cytoskeletal 12 antibody, anti KRT12 antibody This antibody (KG 8. 9 (2017): e0184916. . 14 In this study, CK5/6, CK14, and CK34betaE12 showed similar Immunohistochemistry with cytokeratin 5 and cytokeratin 6 antibodies on sections from dorsal skin tetop-LA G608G+; K5tTA + F1 line VF1-07 with severe epidermal hyperplasia show mislocalized keratin 5 and keratin 6 expression . Validated for Flow, IF and IHC. 100% Guaranteed. Immunogen. There are currently no reviews for Cytokeratin 5 + 6 antibody [D5/16 B4] (ready-to-use) (GTX17068). Cat. Comparing the Diagnostic Outcomes of Staining Various Breast Lesions with Either Anti-CK 5/6 or Anti-CK 5. Do you need  They do this by staining tissue samples with cytokeratin 5/6, an antibody that detects cytokeratins 5 and 6. Cytokeratin 6 Antibody (SPM269) is a mouse monoclonal IgG 2a (kappa light chain) provided at 200 µg/ml raised against a synthetic peptide from the C-terminus of human Cytokeratin 6 recommended for detection of Cytokeratin 6 of mouse, rat and human origin by WB, IP, IF and IHC(P) In Western blotting of cytoskeletal preparations of epidermis and non-keratinizing epithelium, the antibody labels cytokeratin 5. This antibody is effective in immunohistochemistry (IHC). Product Description Studies have shown Cytokeratin 5/6 antibody reacts with human epidermis and non-keratinizing epithelium. CK 5 and 6 can positively be seen in undifferentiated Large-cell Carcinoma as well as Squamous Carcinoma. 1,CytokeRatin 19 Polyclonal Antibody was diluted at 1:200(4°C,overnight). Cytokeratin 6 Antibodies Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Cytokeratin 8, Cytokeratin 10, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16 and Cytokeratin 19. As its name implies, cytokeratin AE1/AE3 is a mixture of two different clones of anti-cytokeratin monoclonal antibodies, AE1 and AE3. Obtain accurate, reproducible results in immunohistochemistry experiments with Thermo Scientific™ Cytokeratin 5/6 Ab-2, Mouse Monoclonal Antibody, one marker in a comprehensive antibody portfolio that addresses all major disease states, ensuring the ideal tests are always available. 3. In our current study, CAM5-2 stained only eight of the 14 cases. Consult the supplier page to verify the identity of the desired antibody target and learn more detailed product information, such as species reactivity Cytokeratin 5 & 6 antibody is positively seen in nearly 100% of Malignant Mesotheliomas and are rarely seen in Lung Adenocarcinomas. Product Description, Rabbit monoclonal to   Anti-Cytokeratin 5 Antibody, 6, clone D5/16B4 is an antibody against Cytokeratin 5 for use in IF, IH(P) & WB. The R-PLEX Human Cytokeratin-8 Antibody Set can be used with other MSD components (see "Recommended Components" tab below) to build assays that measure Cytokeratin-8. Custom antibody services and bulk production also available. Wash twice 5 minutes with buffer A 12. Reacts strongly with cytokeratins 5 and 6 and weakly with cytokeratin 4. In 2006 a new systematic nomenclature for mammalian keratins was created, and the proteins previously called "cytokeratins" are simply called keratins (human Cytokeratin 6 is a type II cytokeratin known for its strong induction in stratified epithelia that features an enhanced cell proliferation rate or abnormal differentiation during wound healing. Array 11 - Chemicals & Heavy Metals. It stains basal cells in combined and stratified epithelial tissues. g. 5 kD. It labels mesothelioma, and epithelial basal cells in prostate and tonsil. Background: Antibodies binds to cytokeratins (4, 5, 6, 8, 10, 13, and 18) of simple epithelium and tumor derivatives and are expressed in stratified squamous epithelium. RRID: AB_2837855 Please cite this product as: Affinity Biosciences Cat# AF5370, RRID:AB_2837855. Point mutations in the cytokeratin 5 gene at locus 12q11-q13 can cause various types of epidermolysis bullosa simplex. Be the first to share your experience with this product. Tested in Human, Mouse. quantity: 100 µL price: 399 USD to the supplier Page: 1 of 6 Cytokeratin Monoclonal FITC Antibody (Clone C-11) SAFETY DATA SHEET Supersedes Revision: 08/01/2019 according to Regulation (EC) No. Three commonly used keratin monoclonal antibodies (MAB)--AE1:AE3, CAM 5. expressed by proliferating squamous epithelium often paired with Cytokeratin 16. McNamara NA PloS one 12. 6 (2018 Jun 1): 2436-2451. Product Name, Anti-CK-5/CK-6 (Cytokeratin 5/ Cytokeratin 6) Rabbit Monoclonal Antibody [RM341]. 5 ug CoraLite®488-conjugated Anti-Human Cytokeratin 19 (CL488-60187, Clone:3G1E4) (green), and 0. Cytokeratin 5/6 cannot identify mesothelioma on its own. This antibody reacts with Human, Mouse, Rat samples. 6   Our main activity is design and production of monospecific clonal antibodies which are uniquely characterized by exceptional specificity, affinity and avidity. Cytokeratin 6 (EP67) Rabbit Monoclonal Antibody Updated: 2017-05-24 08:46:09 The human type II Cytokeratin 6 (CK6; 56 kDa) is well known for its strong induction in stratified epithelia that feature an enhanced cell proliferation rate or abnormal differentiation during wound healing, in several diseases (e. . Product Details. Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0. Fewer than 10% of Carcinomas of the breast, colon, and prostate stain positively for this marker. 2, an antibody specific for cytokeratin 7 and 8, and has been shown to react with native cytokeratin preparations containing cytokeratins 8, 18, and 19 as well as with recombinant cytokeratin 8. Clone LL020 is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 murine myeloma cells with spleen cells from a mouse immunized with a carboxyterminal peptide of cytokeratin 6. View specifications, prices, citations, reviews, and more. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 6 antibody (ab93962) ab93962, at 1/200 dilution, staining Cytokeratin 6 in formalin-fixed, paraffin-embedded Human prostate tissue by Immunohistochemistry. CK5/6 has been shown to be a reliable marker for mesothelioma and squamous cell carcinoma of the lung. Assay Information This Cytokeratin 4+5+6+8+10+13+18 antibody (FITC) is also available in a pre-diluted ready-to-use format expecially for IHC under catalog no. Product Specific Information Where clone OV-TL 12/30 can produce unwanted staining of endothelial cells, clone RN7 does not stain these cell types. Cited in 1 reference(s). 2ug Cytokeratin 8 antibody (17514-1-AP, red) and control antibody (blue). Format: Liquid. Miller, M. The antibody does not cross-react with cytokeratins 1, 7, 8, 10, 13, 14, 18 and 19. CK5/6 expression is a marker used in diagnosing breast cancers in basaloid group and is associated with a poor prognosis. They do this by staining tissue samples with cytokeratin 5/6, an antibody that detects cytokeratins 5 and 6. The Journal of clinical investigation 128. 2ug Cytokeratin 6A antibody (10590-1-AP, red) and control antibody (blue). 1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for half an hour at room temperature. Chemicals and Their Role in the Development of Alzheimer's  Storage, Store at < -20°C, stable for 6 months. mouse anti-keratin antibody, mouse anti-cytokeratin 6 antibody, PC2 antibody, Type II keratin isoform K6c antibody. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0. Please minimize freeze-thaw cycles. PKKl is reported to react with cytokeratin  However, certain key mediators of cytokine release syndrome have been identified. These cytokeratins have been reported to be expressed in tumor cells of epithelial origin and less commonly of mesothelial origin. Antigen Retrieval: Trilogy™ (EDTA based, pH 8. 014 % 231-791-2 Cytokeratin 8/18 Cytokeratin 14 Cytokeratin 15 Cytokeratin 19 Cytokeratin 20 Cytokeratin, High Molecular Weight (CK34BE12) Cytokeratin 5/6 Cytokeratin 7 Claudin-4 Cytomegalovirus c-MYC C-reactive Protein Cyclin D1 Cyclophilin B Delta Catenin Mouse anti human Cytokeratin 14 antibody, clone LL002 (MCA890) used for the demonstration of cytokeratin 14 expressing cells in human buccal epithelium and urothelium by immunohistochemistry on formalin fixed paraffin embedded tissue sections. Cytokeratin 6 Antibody (SPM269) Summary. 1 Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor 647. 0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:400 overnight at 4\u00b0C, DAB staining. Anti-mIL-6-IgG is produced in hybridomas and purified by affinity chromatography. 0) buffer, 15min Anti-Cytokeratin 6/75 Antibody (H-6) is a mouse monoclonal IgG 2a κ Cytokeratin 6/75 antibody, cited in 2 publications, provided at 200 µg/ml; specific for an epitope mapping between amino acids 147-180 within an internal region of Cytokeratin 6A of human origin Cytokeratin 6 Antibody (B-7) is a mouse monoclonal IgG 1 κ, cited in 2 publications, provided at 200 µg/ml specific for an epitope mapping between amino acids 19-34 near the N-terminus of Cytokeratin 6 of human origin Keratin 6 (KRT6), also known as cytokeratin-6 or CK-6, is a type II intermediate filament protein constitutively expressed in all major types of epithelial appendages, such as hair, nail, and glands. Similarly, loss of tumor suppressor gene PTEN and a high expression of c-Met has been associated with poor prognosis Cytokeratin 6A-specific antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP),Enzyme-linked Immunosorbent Assay (ELISA) applications. 914 -99. CK5/6 expression is a marker used in diagnosing breast cancers in basaloid group and is associated with a poor prognosis. Cytokeratin 18 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human Cytokeratin 18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7619) at 3 µg/mL overnight at 4 °C. Compare Anti-Pan cytokeratin Flow Cytometry Antibody Products from leading suppliers on Biocompare. Antibodies Format Concentration Size CF® dye conjugates (13 colors) 0. View PDF In Iranian Journal of Pathology on 19 September 2019 by Gudi, M. Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Cases were scored as positive or negative against a negative control. ABclonal provides trial size antibody samples for target detection. Cytokeratins are represented in epithelial For example, cytokeratin antibody can be used as an epithelial tumoral marker (Krishna, 2010), glial fibrillar acidic protein as a glioblastoma marker (Guichet et al. Cytokeratin 8 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 1. Shipping JavaScript if it is disabled in your browser. 2. Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor® 488 is an antibody against Cytokeratin 5,6 for use in Immunocytochemistry. CK 5 and 6 have also been used successfully as a Rabbit Polyclonal Cytokeratin 6 antibody. Visit Bethyl. Cytokeratin 6 Antibody detects endogenous levels of total Cytokeratin 6. 1% PBS-Triton for 20 min. Tested in Human. 1 Product Result | Match Criteria: Product Name, Description The antibody is a broad spectrum anti-keratin reagent reacting with intermediate and low-molecular-weight keratins. (1) Principle of Procedure: Antigen detection in tissues and cells is a multi-step immunohistochemical process. It stains basal cells in combined and stratified epithelial tissues. 1 Product Result Revision: 03/24/2015 Page: 2 of 6 Cytokeratin Monoclonal Antibody (Clone C-11) SAFETY DATA SHEET Supersedes Revision: 07/16/2010 WC4500000 NA 7732-18-5 ZC0110000 Water 98. Cytokeratins 5 and 6 are also expressed in basal cell epitheliomas, squamous cell carcinomas of skin, tongue, epiglottis and of the rectal-anal region. Cury PM, et al. Anti-Cytokeratin 5/6 (D5/16B4) Mouse Monoclonal Primary Antibody (anti-Cytokeratin 5/6 (D5/16B4)) detects cytokeratins 5 and 6. No. Storage Condition and Buffer: Cytokeratin 18 Monoclonal Antibody (C-04), FITC Catalog Number MA1-10326 Product Data Sheet Background/Target Information Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. The MA1-26237 immunogen is crude extract of splenic cells from a nude mouse engrafted with MCF-7 cells. Unconjugated Whole IgG Mouse anti-Cytokeratin Monoclonal Antibody, Purified [AE1/AE3] suitable for IHC, IHC-IF, ICC-IF, mIF applications. Cytokeratins are intermediate filament proteins   Human cytokeratin 5, 6, 8 and 18 intermediate filament proteins. Less<< Anti-Cytokeratin 10 Antibody, clone RKSE60 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. 1 Product Result Abundant immunostaining was observed in all UDH using both antibodies. 2000 Feb;13(2):107-12. These are used in immunohistochemistry, often called CK 5/6 staining, including the following applications: Identifying basal cells or myoepithelial cells in the breast and prostate. Use of this antibody is indicated, subsequent to clinical differential diagnosis, as an aid in the identification of carcinoma within the context of an antibody panel, the patient’s clinical history, and other diagnostic tests Anti-CK 5 & 6 positivity is seen in 65% to 100% of epithelioid mesotheliomas, in 0% to 19% of lung adenocarcinomas, and in 22% to 80% of serous carcinoma of the ovary and peritoneum. [AE1/AE3] recognizes acidic and basic subfamilies of cytokeratins, with molecular weights ranging from 40 to 67 kDa. Anti-CK-5 (Cytokeratin 5) Rabbit Monoclonal Antibody Data Sheet RM226. com Cytokeratin immunostains will stain most carcinomas, carcinoid tumors, epithelial organs, etc. ARG62969 anti-Cytokeratin (pan) antibody [C-11] ICC/IF image. CK 5 and 6 can positively be seen in undifferentiated Large-cell Carcinoma as well as Squamous Carcinoma. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Keratin 5 & 6 expression in lung adenocarcinoma is not common, and when it occurs, the reaction is usually confined to small focal areas or scattered cells. 2 Epithelioid mesotheliomas are strongly positive in almost all cases, but a Figure 6. 75R-1022 Cytokeratin 12 antibody detects endogenous levels of total Cytokeratin 12. Mouse. See below for cytokeratin antibodies, many with wide reactivity, and based on the expertise of Upstate & Chemicon. rat Keratin, type II/ Cytokeratin 8 Krt8 antibody validated for FACS,FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot Javascript is disabled on your browser. The primary antibody (ET1601-6, 1/500) was used in 5% BSA at room temperature for 2 hours. The initial step binds the primary antibody to its specific epitope. Schäfer H, et al. , 2013), and vimentin as a marker for some sarcomas . 21 mg/mL). 05). We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. KRT6 has been identified as a marker of mammary gland progenitor cells. - Find MSDS or SDS, a COA, data sheets and more information. Histopathology. Mob362-01 | Mouse Monoclonal Antibody to Cytokeratin 5/6 (clone: D5/16 B4) - Concentrated. We conclude that all four antibodies show high sensitivity for epithelioid mesothelioma, but only calretinin (98%), cytokeratin 5/6 (86%), and thrombomodulin (81%) show sufficient specificity for practical use in this situation. 2% of Tween-20 (buffer A) for 5 minutes 10. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. With 34betaE12 antibody, three of five of the ADH cases showed less than 5% labeled cells, while two cases showed more than 30% of stained cells. Cytokeratin 6 antibody LS-C22718 is an unconjugated mouse monoclonal antibody to Cytokeratin 6 (KRT6 / CK6) from human. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. psoriasis, actinic keratosis) and in cancer. Cytokeratins 5, 6 and 18 are reported to be expressed in a broad range of human epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. 1 Cury, P. Reacts strongly with cytokeratins 5 and 6 and weakly with cytokeratin 4. Chief among these is interleukin 6 (IL-6), an activator of the JAK/STAT   Ordering: To place an order via credit card or PO use Add to Cart button (creating an account is not required). Anti-Cytokeratin 5 Antibody, 6, clone D5/16B4 is an antibody against Cytokeratin 5 for use in IF, IH(P) & WB. Cytokeratin 6 Antibody (LL020) [Alexa Fluor® 488] Summary. 05M TRIS/HCl, pH 7. 66146-1-Ig. 6RXUFH Polyclonal Sheep IgG 3XULILFDWLRQ Antigen Affinity­purified pan-cytokeratin; pan-CK; pan CK; P-CK; wide spectrum Cytokeratin; Cytokeratins; [cytokeratins 1, 2,4,5,6,7,8,71,72,75,78]. In subgroups of breast cancer, the shortest disease-free and overall survival was observed in basaloid and human epidermal growth factor receptor-2 groups. It is reactive with human and mouse. IF analysis of Cytokeratin 8 using anti- Cytokeratin 8 antibody (M01421-3). Immunofluorescent staining of Cytokeratin 6 in human cell line HaCaT using a Cytokeratin 6 Polyclonal Antibody (Product # PA5-61074) shows localization to intermediate filaments. It does not react with pulmonary adenocarcinoma. A. Immunohistochemistry with cytokeratin 5 and cytokeratin 6 antibodies on sections from dorsal skin tetop-LA G608G+; K5tTA + F1 line VF1-07 with severe epidermal hyperplasia show mislocalized keratin 5 and keratin 6 expression . Replacement to Abcam, Santa Cruz, Sigma and CST antibody. Please note: Anti-Cytokeratin 5 + 6 Antibody [RM341] is for research use only. Roll over image to zoom in Product code: 36-3776 Anti-cytokeratin antibody is coated on magnetic microbeads and incubated with disseminated cells of metastatic carcinomas. Monoclonal antibodies are available to most protein immunogens. 5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Anti-Cytokeratin 14/KRT14 Mouse Monoclonal Antibody (100129-MM01T), manufactured by Sino Biological is validated in IHC-P. The Hippo signaling functions through the Notch signaling to regulate intrahepatic bile duct development in mammals. Storage Condition and Buffer: As I am sure you are aware, the antibody purchased, Anti-Cytokeratin 5 + 6 antibody (ab17133) was ordered quite a long time ago. CK OSCAR is the preferred antibody for distinguishing neoplastic spindle cells from reactive myofibroblasts. Cat. 100% Guaranteed. In adult tissues cytokeratin 8 is expressed as a heterodimer with cytokeratin 18. Cytokeratin 19 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB3506) at 10 µg/mL for 3 hours at room temperature. Clone REA885 recognizes the human type II keratin protein cytokeratin 8, an intermediate filament protein, which is found in epithelial and carcinoma cells. Expression of KRT6 is induced by cell injury, infection, and disease. . Anti-Cytokeratin 5 & 6 stains the cytoplasm of such cells. 1-3 Anti-Cytokeratin 5 & 6 is a useful immunohistochemical marker in the identification of mesothelioma and lung squamous cell carcinoma. Aire-deficient mice provide a model of corneal and lacrimal gland neuropathy in Sjögren's syndrome. Mouse anti Human Cytokeratin (Pan) antibody, clone AE1/AE3 is a pan-cytokeratin reagent, being a cocktail of clones AE1 and AE3 which provides the broadest spectrum of reactivity to the 19 known human epidermal keratins. It shows a broad pattern of reactivity with human epithelial tissues from simple glandular epithelial to stratified squamous epithelia, like Pan Cytokeratin antibody Plus is a combination of [AE1/AE3] and Cytokeratin (CK) 8/18 [5D3] and can be used to detect most human epithelia. Negative control was used by secondary antibody only. Antibody to Cytokeratin 7 strongly stains all cell layers of the urinary bladder transitional epithelium. It recognizes the stem cell population, including the so-called amplifying cell in the prostate epithelium. Both the CK7-/CK20+ phenotype and expression of the antibody CDX2 are highly specific and sensitive markers of colorectal origin. This MAb recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). Antibodies Indicate Body Burden. Monoclonal Anti-Cytokeratin, pan−FITC antibody produced in mouse. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. No. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. RM226. View specifications, prices, citations, reviews, and more. The Cytokeratin, High Molecular Weight (Clone 34βE12) antibody reacts primarily with complex epithelium such as squamous, ductal and transitional epithelium and corresponding tumors. ARG66385 anti-Cytokeratin 6 antibody [SQab18112] ICC/IF image. Antibody [PA5-16722] Cytokeratin 14 Polyclonal Antibody by Invitrogen Antibodies. The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. Cytokeratin 5 & 6 (D5/16B4) + TTF-1 (8G7G3/1) Mouse Monoclonal Antibody. Validated for IHC and tested on formalin fixed,  Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor 555 clone D5/16B4, from mouse, ALEXA FLUOR® 555; Synonym: Keratin, type II cytoskeletal 5,  Anti-Cytokeratin 5 & 6 is a useful immunohistochemical marker in the identification of mesothelioma and lung squamous cell carcinoma. It recognizes the stem cell population, including the so-called amplifying cell in the prostate epithelium. To view this site, you must enable JavaScript or upgrade to a JavaScript-capable browser. Return to top. In addition, IgG antibody-induced cytotoxicity was significantly inhibited when IgG antibodies from patients with nonallergic asthma were pre-incubated with recombinant human airway epithelial cell autoantigens (cytokeratin 18 or alpha-enolase proteins; p < 0. Cytokeratin 6 antibody (GTX105355) diluted at 1:500. Western blot analysis of Cytokeratin 6 expression in A431 cell lysate (M30943-1). Cytokeratin 5 can be useful in the distinction of mesotheliomas from most adenocarcinomas. Cytokeratin 5 & 6 antibody has also been used successfully KRT6/1702 is a Mouse Monoclonal Antibody to Cytokeratin 6. What is its role in COVID-19? Cytokeratin 1/5/10/14 (human) monoclonal antibody (34βE12) - ENZ-C34903. Cytokeratin 4+5+6+8+10+13+18 antibody was raised in Mouse using Keratin preparation from human cultured cell line A431 as the immunogen. Format: Liquid. KA12] (ab18586) Overlay histogram showing A431 cells stained with ab18586 (red line). CK5/6 expression is a marker used in diagnosing breast cancers in basaloid group and is associated with a poor prognosis. The recommened dilution for this antibody in western blot applications is 1:25. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6. Angew Chem Int Ed Recently, a p40 antibody was suggested to be a highly specific marker for SQC. Antibodies to this protein identify basal cells of squamous and glandular epithelia, myoepithelia, and mesothelium. Clone CK 223 Isotype IgG1 kappa; Alternatives (show) This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. Antibody manufacturer - Antibodies, Antibody, Antibody Suppliers, Antibody Database, Antibody Catalog The cytokeratin family of proteins is encoded by 30 genes MAB3224 is a broadly reacting cytokeratin antibody which reacts with cytokeratin 5 among others. It also labels cytokeratin 6 and weakly labels cytokeratin 4. This antibody reacts Cytokeratin 5, Cytokeratin 6, Cytokeratin 8, Cytokeratin 17 and Cytokeratin 19. Cytokeratin 5 & 6 antibody is positively seen in nearly 100% of Malignant Mesotheliomas and is rarely seen in Lung Adeno-carcinomas. No cross-reaction with cytokeratins 1, 7, 8, 10, 13/14, 18 and 19 has been found. Phone: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: [email protected] Description. In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. Value of the mesothelium-associated antibodies thrombomodulin, cytokeratin 5/6, calretinin, and CD44H in distinguishing epithelioid pleural mesothelioma from adenocarcinoma metastatic to the pleura. Mod Pathol. 100% Guaranteed. Custom antibody services and bulk production also available. Rabbit Monoclonal Anti-Cytokeratin 6 Antibody (SP87). 7 µg/mL overnight at 4 °C. OSCAR is reactive with most epithelial types (in normal tissues) and with most carcinomas. Cytokeratin 19 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human Cytokeratin 19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3506) at 10 µg/mL for 3 hours at room temperature. Proteintech Anti-Cytokeratin 6A Polyclonal, Catalog # 10590-1-AP. Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. Cytokeratin 8 was detected in immunocytochemical section of U20S cell. ABclonal provides free trial antibodies for target detection. Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620) at 1/100000 dilution + A431 cell lysate at 10 µg Secondary Goat anti-rabbit HRP antibody at 1/2000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa Synthetic peptide within Human Cytokeratin 6 aa 1-100 (N terminal). Cytokeratin 18 in Human Breast Cancer Tissue. It is not intended for diagnostic or therapeutic use. Concentration: 1mg/ml. CK 5 and 6 can positively be seen in undifferentiated Large-cell Carcinoma as well as Squamous Carcinoma. Background: Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The term "cytokeratin" began to be used in the late 1970s, when the protein subunits of keratin intermediate filaments inside cells were first being identified and characterized. View specifications, prices, citations, reviews, and more. Cytokeratin 6 antibody can be used to differentiate Mesothelioma (positive) from Lung Carcinoma (negative) or metastatic carcinoma (negative), and can also be used to distinguish between Ductal Hyperplasia of the breast (positive) from Solid Papillary DCIS (negative). Keratins play a critical role in mechanical stability and integrity of epithelial cells and tissues, have regulatory functions, and are involved in Cytokeratin 5 and 6 are expressed in basal cell epithelioma, basal cells of prostate, urothelium, vagina, squamous cell carcinomas of skin, tongue, epiglottis and rectal-anal region. The Cytokeratin 5/6 antibody is a mouse monoclonal antibody targeting human Cytokeratin5/6. The primary antibody (EM1901-08, 1/100) was used in 5% BSA at room temperature for 2 hours. Applications: WB and IHC. These are used in immunohistochemistry, often called CK 5/6 staining, including the following applications: Identifying basal cells or myoepithelial cells in the breast and prostate. M. The antibody does not cross-react with cytokeratins 1, 7, 8, 10, 13, 14, 18 and 19. Description: The monoclonal antibody LL020 recognizes human Cytokeratin 6. Incubate the section with primary antibody diluted in buffer A at the dilution 1:100-1:200 for 1 hour in the closed wet chamber 11. Transfection Reagent for Beta-TC-6 Cells (Pancreas   Diagnostic Kit for Antibody to Helicobacter Pylori, HP-Ab, 1000 Kit for Cardiac Troponin I,MYO,Isoenzyme MB of Creatine Kinase, cTnI, MYO, CK-MB, 10000. ABclonal provides trial size antibody samples for target detection. This protein A purified  MAK-6 is a com- bination antibody recognizing cytokeratins of molecular weights ranging from 40 to 52. Cytokeratin 5 is expressed in various epithelia and mesothelial cells, while cytokeratin 6 is expressed by proliferating squamous epithelium. MAB1620) is shown to be suitable also for immunofluorescence, immunohistochemistry, and Western blotting applications. Purification Purified by Protein A Chromatography Immunogen Cytokeratin 4+5+6+8+10+13+18 antibody was raised in Mouse using Keratin preparation from human cultured cell line A431 as the immunogen. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56. MA1-26237 detects Cytokeratin from human samples. 2, and MAK-6--were compared with routinely used cytokeratin antibody. Fewer than 10% of Carcinomas of the breast, colon, and prostate stain positively for this marker. 2, Sodium citrate pH 6. Cytokeratin 6 (10F4) Monoclonal Antibody: Applications: WB, IHC-P, IF(ICC), IHC: Reactivity: Human: Specifications: Conjugation: Unconjugated: Host: Rabbit: Source: N terminus human Cytokeratin 6: Clonality: Monoclonal: Clone # 10F4: Isotype: IgG: Concentration: 1ug/ul: Purification: Purified by Protein A. Useful in differentiating sarcomatoid carcinoma from sarcoma. - Find MSDS or SDS, a COA, data sheets and more  Buy rabbit monoclonal (RM341) antibody to Cytokeratin 5 + 6 (A121367). As such, it can be used as a marker of squamous differentiation Western blot analysis of Cytokeratin 5+6 on HT-29 cell lysates. This antibody has been selected for its ability to efficiently neutralize the biological activity of mIL-6. Acquisitions of 20,000 events were performed. Rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7. Image caption: Comparison between buccal epithelium and urothelium. View All Images (3) Cytokeratin 6 is located in proliferating squamous epithelium, whereas Cytokeratin 5 is found in the basal, the intermediate and the superficial cell layers of stratified and transitional epithelia, complex epithelia, and in mesothelial cells and mesothelioma, but not in simple epithelia and in non-epithelial cells. This antibody reacts with Human, Mouse. Keratins play a critical role in mechanical stability and integrity of epithelial cells and tissues, have regulatory functions, and are involved in Cytokeratin 8 in Human Colon Cancer Tissue. Immunohistochemical analysis of paraffin-embedded Lung CA using Cytokeratin 14 (PA5-28002) antibody at 1:100 dilution. Contents. Rabbit Cytokeratin 14 Rabbit mAb (A19039), validaed in WB,, IHC and tested in Human,, Mouse,, Rat. 00 USD. cytokeratin 6 antibody


Cytokeratin 6 antibody